Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy.

Details

Ressource 1Request a copy Sous embargo indéterminé.
State: Public
Version: author
Serval ID
serval:BIB_94D873A596E0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy.
Journal
Journal of Biomedical Optics
Author(s)
Boss D., Kühn J., Jourdain P., Depeursinge C., Magistretti P.J., Marquet P.
ISSN
1560-2281 (Electronic)
ISSN-L
1083-3668
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
18
Number
3
Pages
36007
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
ABSTRACT. A dual-wavelength digital holographic microscope to measure absolute volume of living cells is proposed. The optical setup allows us to reconstruct two quantitative phase contrast images at two different wavelengths from a single hologram acquisition. When adding the absorbing dye fast green FCF as a dispersive agent to the extracellular medium, cellular thickness can be univocally determined in the full field of view. In addition to the absolute cell volume, the method can be applied to derive important biophysical parameters of living cells including osmotic membrane water permeability coefficient and the integral intracellular refractive index (RI). Further, the RI of transmembrane flux can be determined giving an indication about the nature of transported solutes. The proposed method is applied to cultured human embryonic kidney cells, Chinese hamster ovary cells, human red blood cells, mouse cortical astrocytes, and neurons.
Pubmed
Web of science
Create date
26/03/2013 10:38
Last modification date
20/08/2019 15:57
Usage data