Isolation and characterization of Marek's disease virus (MDV) cDNAs mapping to the BamHI-I2, BamHI-Q2, and BamHI-L fragments of the MDV genome from lymphoblastoid cells transformed and persistently infected with MDV.

Details

Serval ID
serval:BIB_94385EBB9D26
Type
Article: article from journal or magazin.
Collection
Publications
Title
Isolation and characterization of Marek's disease virus (MDV) cDNAs mapping to the BamHI-I2, BamHI-Q2, and BamHI-L fragments of the MDV genome from lymphoblastoid cells transformed and persistently infected with MDV.
Journal
Virology
Author(s)
Peng Q., Zeng M., Bhuiyan Z.A., Ubukata E., Tanaka A., Nonoyama M., Shirazi Y.
ISSN
0042-6822 (Print)
ISSN-L
0042-6822
Publication state
Published
Issued date
10/11/1995
Peer-reviewed
Oui
Volume
213
Number
2
Pages
590-599
Language
english
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Abstract
We have isolated and sequenced two cDNAs of sizes 2674 and 677 bp from a cDNA library derived from MKT-1, a lymphoblastoid cell line transformed and latently infected with Marek's disease virus (MDV) using probes corresponding to the right-hand end of the BamHI-I2 fragment of the MDV genome. The larger cDNA clone represents an abundant transcript, which extends from the right-hand end of BamHI-I2 to the adjacent BamHI-Q2 and BamHI-L fragments of the MDV genome and contains the Meq (MDV Eco-Q) open reading frame. The smaller cDNA clone represents a spliced transcript containing the putative DNA binding domain of Meq as well as sequences in the BamHI-L region. We prepared a polyclonal antibody against part of the protein sequence of Meq and detected a 44-kDa protein in MKT-1 cells and in cells lytically infected with MDV. In addition, riboprobes corresponding to sequences specific to each cDNA as well as shared sequences between cDNAs detected a number of transcripts in cells either lytically or latently infected with MDV. Our results indicate that the Meq transcriptional unit extends to the BamHI-L fragment and that the transcripts mapping to the right-hand end of the BamHI-I2 and adjacent BamHI-Q2 and BamHI-L fragments are not preferentially expressed during latency.

Keywords
Amino Acid Sequence, Animals, Base Sequence, Cell Transformation, Viral, DNA Primers, DNA, Complementary/isolation & purification, DNA, Viral/genetics, DNA, Viral/isolation & purification, Deoxyribonuclease BamHI, Gene Library, Genome, Viral, Herpesvirus 2, Gallid/genetics, Herpesvirus 2, Gallid/physiology, Lymphocytes/virology, Molecular Sequence Data, RNA, Viral/genetics, Restriction Mapping, Transcription, Genetic, Tumor Cells, Cultured, Viral Proteins/analysis, Viral Proteins/genetics, Virus Latency
Pubmed
Web of science
Open Access
Yes
Create date
01/03/2018 16:41
Last modification date
20/08/2019 15:56
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