Efficient induction of tumor antigen-specific CD8+ memory T cells by recombinant lentivectors
Details
Serval ID
serval:BIB_93A1C347FBDC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Efficient induction of tumor antigen-specific CD8+ memory T cells by recombinant lentivectors
Journal
Cancer Research
ISSN
0008-5472 (Print)
Publication state
Published
Issued date
01/2006
Volume
66
Number
2
Pages
1155-60
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan 15
Research Support, Non-U.S. Gov't --- Old month value: Jan 15
Abstract
The success of active cancer immunotherapy entails a robust induction of tumor-reactive effector and memory CD8+ T cells. We compared the in vivo immunogenicity of the melanoma-associated antigen Melan-A(26-35) encoded by third-generation recombinant lentivector (rec. lv) or as peptide admixed with a strong adjuvant. Ex vivo analyses of immunized HLA-A2/H-2K(b) mice showed that rec. lv triggered a stronger anti-Melan-A CD8+ T -cell response than peptide vaccine. Importantly, the majority of anti-Melan-A T cells elicited by rec. lv expressed the memory marker CD127 at the peak of the primary response. In those mice, memory T cells were detectable several months after priming and could be activated by recall peptide vaccination. These results show that immunization with rec. lv induces not only a strong antigen-specific CD8+ T -cell response but also a long-lasting T-cell memory against a bona fide tumor-associated antigen.
Keywords
Animals
Antibody Formation
Antigens, Neoplasm/immunology
CD8-Positive T-Lymphocytes/*immunology
Cancer Vaccines/*immunology
Genetic Vectors
HLA-A2 Antigen/genetics
Immunologic Memory/*immunology
Lentivirus/genetics
Melanoma/immunology
Mice
Mice, Transgenic
Neoplasm Proteins/immunology
Peptide Fragments
Skin Neoplasms/immunology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 11:17
Last modification date
20/08/2019 14:56