Taxol and tau overexpression induced calpain-dependent degradation of the microtubule-destabilizing protein SCG10

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Version: Author's accepted manuscript
Serval ID
serval:BIB_924545418DFF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Taxol and tau overexpression induced calpain-dependent degradation of the microtubule-destabilizing protein SCG10
Journal
Experimental Neurology
Author(s)
Vega I.E., Hamano T., Propost J.A., Grenningloh G., Yen S.H.
ISSN
0014-4886 (Print)
1090-2430 (Electronic)
ISSN-L
0014-4886
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
202
Number
1
Pages
152-160
Language
english
Notes
Publication types: Article ; research-article Identifiant PubMed Central: PMC3696491
Abstract
Microtubule-stabilizing and -destabilizing proteins play a crucial role in regulating the dynamic instability of microtubules during neuronal development and synaptic transmission. The microtubule-destabilizing protein SCG10 is a neuron-specific protein implicated in neurite outgrowth. The SCG10 protein is significantly reduced in mature neurons, suggesting that its expression is developmentally regulated. In contrast, the microtubule-stabilizing protein tau is expressed in mature neurons and its function is essential for the maintenance of neuronal polarity and neuronal survival. Thus, the establishment and maintenance of neuronal polarity may down-regulate the protein level/function of SCG10. In this report, we show that treatment of PC12 cells and neuroblastoma cells with the microtubule-stabilizing drug Taxol induced a rapid degradation of the SCG10 protein. Consistently, overexpression of tau protein in neuroblastoma cells also induced a reduction in SCG10 protein levels. Calpain inhibitor MDL-28170, but not caspase inhibitors, blocked a significant decrease in SCG10 protein levels. Collectively, these results indicate that tau overexpression and Taxol treatment induced a calpain-dependent degradation of the microtubule-destabilizing protein SCG10. The results provide evidence for the existence of an intracellular mechanism involved in the regulation of SCG10 upon microtubule stabilization.
Keywords
Blotting, Western/methods, Calpain/metabolism, Cysteine Proteinase Inhibitors/pharmacology, Enzyme Activation/drug effects, Gene Expression Regulation/drug effects, Leupeptins/pharmacology, Membrane Proteins/metabolism, Paclitaxel/pharmacology, Transfection/methods, Tubulin Modulators/pharmacology, tau Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
11/07/2016 10:04
Last modification date
20/08/2019 14:55
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