Marker-independent identification of glioma-initiating cells.

Details

Serval ID
serval:BIB_91555CC6F3C8
Type
Article: article from journal or magazin.
Collection
Publications
Title
Marker-independent identification of glioma-initiating cells.
Journal
Nature methods
Author(s)
Clément V., Marino D., Cudalbu C., Hamou M.F., Mlynarik V., de Tribolet N., Dietrich P.Y., Gruetter R., Hegi M.E., Radovanovic I.
ISSN
1548-7105 (Electronic)
ISSN-L
1548-7091
Publication state
Published
Issued date
03/2010
Peer-reviewed
Oui
Volume
7
Number
3
Pages
224-228
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Retracted Publication
Publication Status: ppublish
Abstract
Tumor-initiating cells with stem cell properties are believed to sustain the growth of gliomas, but proposed markers such as CD133 cannot be used to identify these cells with sufficient specificity. We report an alternative isolation method purely based on phenotypic qualities of glioma-initiating cells (GICs), avoiding the use of molecular markers. We exploited intrinsic autofluorescence properties and a distinctive morphology to isolate a subpopulation of cells (FL1(+)) from human glioma or glioma cultures. FL1(+) cells are capable of self-renewal in vitro, tumorigenesis in vivo and preferentially express stem cell genes. The FL1(+) phenotype did not correlate with the expression of proposed GIC markers. Our data propose an alternative approach to investigate tumor-initiating potential in gliomas and to advance the development of new therapies and diagnostics.

Keywords
AC133 Antigen, Animals, Antigens, CD/analysis, Biomarkers, Tumor/analysis, Brain Neoplasms/pathology, Cell Differentiation, Cells, Cultured, Fluorescence, Gene Expression Profiling, Glioma/pathology, Glycoproteins/analysis, Humans, Mice, Neoplastic Stem Cells/pathology, Peptides/analysis
Pubmed
Web of science
Create date
16/03/2010 14:52
Last modification date
03/03/2018 19:28
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