Caloric dose-responsive genes in blood cells differentiate the metabolic status of obese men.

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Serval ID
serval:BIB_909371E0BCFF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Caloric dose-responsive genes in blood cells differentiate the metabolic status of obese men.
Journal
The Journal of Nutritional Biochemistry
Author(s)
Gille D., Zangger N., Soneson C., Bütikofer U., Delorenzi M., Schwander F., Kopf-Bolanz K.A., Chollet M., Walther B., Laederach K., Vergères G.
ISSN
1873-4847 (Electronic)
ISSN-L
0955-2863
Publication state
Published
Issued date
2017
Peer-reviewed
Oui
Volume
43
Pages
156-165
Language
english
Abstract
We have investigated the postprandial transcriptional response of blood cells to increasing caloric doses of a meal challenge to test whether the dynamic response of the human organism to the ingestion of food is dependent on metabolic health. The randomized crossover study included seven normal weight and seven obese men consuming three doses (500/1000/1500 kcal) of a high-fat meal. The blood cell transcriptome was measured before and 2, 4, and 6 h after meal ingestion (168 samples). We applied univariate and multivariate statistics to investigate differentially expressed genes in both study groups. We identified 624 probe sets that were up- or down-regulated after the caloric challenge in a dose-dependent manner. These transcripts were most responsive to the 1500 kcal challenge in the obese group and were associated with postprandial insulin and oxidative phosphorylation. Furthermore, the data revealed a separation of the obese group into individuals whose response was close to the normal weight group and individuals with a transcriptional response indicative of a loss of metabolic flexibility. The molecular signature provided by the postprandial transcriptomic response of blood cells to increasing caloric doses of a high-fat meal challenge may represent a sensitive way to evaluate the qualitative impact of food on human health.
Keywords
Dose–Response, Gene expression, High-fat meal, Insulin, Oxidative phosphorylation
Pubmed
Web of science
Open Access
Yes
Create date
28/03/2017 18:06
Last modification date
04/05/2021 6:36
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