Isolation and characterization of major histocompatibility complex (MHC) class II B genes in the Barn owl (Aves: Tyto alba)

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Serval ID
serval:BIB_8EED7062E209
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Isolation and characterization of major histocompatibility complex (MHC) class II B genes in the Barn owl (Aves: Tyto alba)
Journal
Immunogenetics
Author(s)
Burri R., Niculita-Hirzel Hélène, Roulin A., Fumagalli L.
ISSN
0093-7711
Publication state
Published
Issued date
2008
Peer-reviewed
Oui
Volume
60
Number
9
Pages
543-550
Language
english
Abstract
We isolated major histocompatibility complex class II B (MHCIIB) genes in the Barn owl (Tyto alba). A PCR-based approach combined with primer walking on genomic and complementary DNA as well as Southern blot analyses revealed the presence of two MHCIIB genes, both being expressed in spleen, liver, and blood. Characteristic structural features of MHCIIB genes as well as their expression and high non-synonymous substitution rates in the region involved in antigen binding suggest that both genes are functional. MHC organization in the Barn owl is simple compared to passerine species that show multiple duplications, and resembles the minimal essential MHC of chicken.
Keywords
Amino Acid Sequence, Animals, Evolution, Molecular, Histocompatibility Antigens Class II/genetics, Molecular Sequence Data, Strigiformes/genetics
Pubmed
Web of science
Open Access
Yes
Create date
19/05/2008 15:06
Last modification date
01/10/2019 7:18
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