Disuse deterioration of human skeletal muscle challenged by resistive exercise superimposed with vibration: evidence from structural and proteomic analysis.

Details

Serval ID
serval:BIB_8E7118AB14A9
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Disuse deterioration of human skeletal muscle challenged by resistive exercise superimposed with vibration: evidence from structural and proteomic analysis.
Journal
Faseb Journal : Official Publication of the Federation of American Societies For Experimental Biology
Author(s)
Salanova M., Gelfi C., Moriggi M., Vasso M., Viganò A., Minafra L., Bonifacio G., Schiffl G., Gutsmann M., Felsenberg D., Cerretelli P., Blottner D.
ISSN
1530-6860 (Electronic)
ISSN-L
0892-6638
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
28
Number
11
Pages
4748-4763
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
In the present bed rest (BR) study, 23 volunteers were randomized into 3 subgroups: 60 d BR control (Ctr); BR with resistive exercise (RE; lower-limb load); and resistive vibration exercise (RVE; RE with superimposed vibration). The aim was to analyze by confocal and electron microscopy the effects of vibration on myofibril and filament integrity in soleus (Sol) and vastus lateralis (VL) muscle; differential proteomics of contractile, cytoskeletal, and costameric proteins (TN-C, ROCK1, and FAK); and expression of PGC1α and atrophy-related master genes MuRF1 and MuRF2. RVE (but not RE) preserved myofiber size and phenotype in Sol and VL by overexpressing MYBPC1 (42%, P ≤ 0.01), WDR1 (39%, P ≤ 0.01), sarcosin (84%, P ≤ 0.01), and CKM (20%, P ≤ 0.01) and prevented myofibrillar ultrastructural damage as detectable by MuRF1 expression. In Sol, cytoskeletal and contractile proteins were normalized by RVE, and TN-C increased (59%, P ≤ 0.01); the latter also with RE (108%, P ≤ 0.01). In VL, the outcomes of both RVE (acting on sarcosin and desmin) and RE (by way of troponinT-slow and MYL2) were similar. RVE appears to be a highly efficient countermeasure protocol against muscle atrophy and ultrastructural and molecular dysregulation induced by chronic disuse.
Keywords
Adult, Bed Rest/methods, Exercise Therapy, Humans, Male, Middle Aged, Muscle Contraction/physiology, Muscle, Skeletal/metabolism, Muscle, Skeletal/pathology, Muscular Atrophy/metabolism, Muscular Atrophy/therapy, Proteomics, Vibration, Young Adult
Pubmed
Web of science
Create date
18/10/2016 16:07
Last modification date
20/10/2020 8:19
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