Endogenous glutathione levels modulate the frequency of both spontaneous and long wavelength ultraviolet induced mutations in human cells

Details

Ressource 1Download: serval:BIB_8BF899F5DFF5.P001 (404.15 [Ko])
State: Public
Version: author
License: Not specified
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
Serval ID
serval:BIB_8BF899F5DFF5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Endogenous glutathione levels modulate the frequency of both spontaneous and long wavelength ultraviolet induced mutations in human cells
Journal
Carcinogenesis
Author(s)
Applegate  L. A., Lautier  D., Frenk  E., Tyrrell  R. M.
ISSN
0143-3334 (Print)
Publication state
Published
Issued date
1992
Volume
13
Number
9
Pages
1557-1560
Notes
DA - 19921029
LA - eng
PT - Journal Article
PT - Research Support, Non-U.S. Gov't
RN - 0 (Radiation-Sensitizing Agents)
RN - 1982-67-8 (Methionine Sulfoximine)
RN - 5072-26-4 (Buthionine Sulfoximine)
RN - 70-18-8 (Glutathione)
RN - EC 2.4.2.8 (Hypoxanthine Phosphoribosyltransferase)
SB - IM
Abstract
Spontaneous and induced mutations at the hypoxanthine guanine phosphoribosyl transferase locus have been measured in cultured human lymphoblastoid (TK6) cell populations under conditions in which cellular glutathione has been severely depleted by overnight treatment with buthionine-S,R-sulfoximine. At maximum levels of glutathione depletion, the increase in spontaneous frequency is at least 5-fold, a finding consistent with the possibility that cellular redox state can modulate the levels of pre-mutagenic damage arising as a result of normal metabolism in cultured human cells. Glutathione depletion does not lead to a significant enhancement in the frequency of mutants that arise as a result of irradiation at 313 nm but does lead to a 3-fold increase in mutations resulting from irradiation at 365 nm. These results indicate that glutathione may quench reactive intermediates that would otherwise lead to spontaneous mutations as well as a fraction of UVA radiation-induced premutagenic damage
Keywords
analogs & derivatives/Buthionine Sulfoximine/Cells/Cells,Cultured/deficiency/genetics/Glutathione/Humans/Hypoxanthine Phosphoribosyltransferase/metabolism/Methionine Sulfoximine/Mutation/pharmacology/physiology/Radiation-Sensitizing Agents/Ultraviolet Rays
Pubmed
Web of science
Open Access
Yes
Create date
18/02/2008 18:33
Last modification date
01/10/2019 7:18
Usage data