Simultaneous identification of multiple mammalian species from mixed forensic samples based on mtDNA control region length polymorphism

Details

Serval ID
serval:BIB_89743BE7C3BA
Type
Article: article from journal or magazin.
Publication sub-type
Case report (case report): feedback on an observation with a short commentary.
Collection
Publications
Title
Simultaneous identification of multiple mammalian species from mixed forensic samples based on mtDNA control region length polymorphism
Journal
Forensic Science International : Genetics Supplement Series
Author(s)
Fumagalli L., Jan Cabrita C., Castella V.
ISSN
1875-1768
Publication state
Published
Issued date
2009
Peer-reviewed
Oui
Volume
2
Number
1
Pages
302-303
Language
english
Abstract
Molecular species identification in mixed or contaminated biological material has always been problematic. We developed a simple and accurate method for mammal DNA identification in mixtures, based on interspecific mitochondrial DNA control region length polymorphism. Contrary to other published methods dealing with species mixtures, our protocol requires a single universal primer pair and amplification step, and is not based on a pre-defined panel of species. This protocol has been routinely employed by our laboratory for species identification in dozens of human and animal forensic caseworks. Six representative forensic caseworks involving the specific identification of mixed animal samples are reported in this paper, in order to demonstrate the applicability and usefulness of the method.
Keywords
DNA mixtures, Species identification, Wildlife forensics, Mitochondrial DNA control region
Create date
17/08/2009 15:19
Last modification date
20/08/2019 14:48
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