In vitro and in vivo ocular biocompatibility of electrospun poly(ɛ-caprolactone) nanofibers.

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Version: Author's accepted manuscript
Serval ID
serval:BIB_88212B28C49F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
In vitro and in vivo ocular biocompatibility of electrospun poly(ɛ-caprolactone) nanofibers.
Journal
European Journal of Pharmaceutical Sciences
Author(s)
Da Silva G.R., Lima T.H., Oréfice R.L., Fernandes-Cunha G.M., Silva-Cunha A., Zhao M., Behar-Cohen F.
ISSN
1879-0720 (Electronic)
ISSN-L
0928-0987
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
73
Pages
9-19
Language
english
Abstract
Biocompatibility is a requirement for the development of nanofibers for ophthalmic applications. In this study, nanofibers were elaborated using poly(ε-caprolactone) via electrospinning. The ocular biocompatibility of this material was investigated. MIO-M1 and ARPE-19 cell cultures were incubated with nanofibers and cellular responses were monitored by viability and morphology. The in vitro biocompatibility revealed that the nanofibers were not cytotoxic to the ocular cells. These cells exposed to the nanofibers proliferated and formed an organized monolayer. ARPE-19 and MIO-M1 cells were capable of expressing GFAP, respectively, demonstrating their functionality. Nanofibers were inserted into the vitreous cavity of the rat's eye for 10days and the in vivo biocompatibility was investigated using Optical Coherence Tomography (OCT), histology and measuring the expression of pro-inflammatory genes (IL-1β, TNF-α, VEGF and iNOS) (real-time PCR). The OCT and the histological analyzes exhibited the preserved architecture of the tissues of the eye. The biomaterial did not elicit an inflammatory reaction and pro-inflammatory cytokines were not expressed by the retinal cells, and the other posterior tissues of the eye. Results from the biocompatibility studies indicated that the nanofibers exhibited a high degree of cellular biocompatibility and short-term intraocular tolerance, indicating that they might be applied as drug carrier for ophthalmic use.
Pubmed
Web of science
Open Access
Yes
Create date
31/03/2015 15:28
Last modification date
20/08/2019 14:47
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