Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation.

Details

Serval ID
serval:BIB_87B7A5F8B553
Type
Article: article from journal or magazin.
Collection
Publications
Title
Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Nawrath C., Poirier Y., Somerville C.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Publication state
Published
Issued date
1994
Volume
91
Number
26
Pages
12760-12764
Language
english
Abstract
In the bacterium Alcaligenes eutrophus, three genes encode the enzymes necessary to catalyze the synthesis of poly[(R)-(-)-3-hydroxybutyrate] (PHB) from acetyl-CoA. In order to target these enzymes into the plastids of higher plants, the genes were modified by addition of DNA fragments encoding a pea chloroplast transit peptide, a constitutive plant promoter, and a poly(A) addition sequence. Each of the modified bacterial genes was introduced into Arabidopsis thaliana by Agrobacterium-mediated transformation, and plants containing all three genes were obtained by sexual crosses. These plants accumulated PHB up to 14% of the dry weight as 0.2- to 0.7-micron granules within plastids. In contrast to earlier experiments in which expression of the PHB biosynthetic pathway in the cytoplasm led to a deleterious effect on growth, expression of the PHB biosynthetic pathway in plastids had no obvious effect on the growth or fertility of the transgenic plants and resulted in a 100-fold increase in the amount of PHB that accumulated. We conclude that there does not appear to be any biological barrier to high-level production of PHB in higher plants. The high level of PHB accumulation also suggests that the synthesis of plastid acetyl-CoA is regulated by a mechanism which responds to metabolic demand.
Keywords
Acyltransferases/metabolism, Aldehyde Oxidoreductases/metabolism, Amino Acid Sequence, Arabidopsis, Cell Compartmentation, Molecular Sequence Data, Plants, Genetically Modified, Plastids/metabolism, Polyesters/metabolism, Recombinant Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 20:49
Last modification date
08/05/2019 21:30
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