Identification of proteins from Plasmodium falciparum that are homologous to reticulocyte binding proteins in Plasmodium vivax.

Details

Serval ID
serval:BIB_8782FE7AEBB9
Type
Article: article from journal or magazin.
Collection
Publications
Title
Identification of proteins from Plasmodium falciparum that are homologous to reticulocyte binding proteins in Plasmodium vivax.
Journal
Infection and Immunity
Author(s)
Triglia T., Thompson J., Caruana S.R., Delorenzi M., Speed T., Cowman A.F.
ISSN
0019-9567 (Print)
ISSN-L
0019-9567
Publication state
Published
Issued date
2001
Volume
69
Number
2
Pages
1084-1092
Language
english
Abstract
Plasmodium falciparum infections can be fatal, while P. vivax infections usually are not. A possible factor involved in the greater virulence of P. falciparum is that this parasite grows in red blood cells (RBCs) of all maturities whereas P. vivax is restricted to growth in reticulocytes, which represent only approximately 1% of total RBCs in the periphery. Two proteins, expressed at the apical end of the invasive merozoite stage from P. vivax, have been implicated in the targeting of reticulocytes for invasion by this parasite. A search of the P. falciparum genome databases has identified genes that are homologous to the P. vivax rbp-1 and -2 genes. Two of these genes are virtually identical over a large region of the 5' end but are highly divergent at the 3' end. They encode high-molecular-mass proteins of >300 kDa that are expressed in late schizonts and localized to the apical end of the merozoite. To test a potential role in merozoite invasion of RBCs, we analyzed the ability of these proteins to bind to mature RBCs and reticulocytes. No binding to mature RBCs or cell preparations enriched for reticulocytes was detected. We identified a parasite clone that lacks the gene for one of these proteins, showing that the gene is not required for normal in vitro growth. Antibodies to these proteins can inhibit merozoite invasion of RBCs.
Keywords
Amino Acid Sequence, Animals, Erythrocytes/metabolism, Erythrocytes/parasitology, Hemoglobins/analysis, Humans, Mice, Molecular Sequence Data, Plasmodium falciparum/chemistry, Plasmodium falciparum/genetics, Plasmodium vivax/chemistry, Protozoan Proteins/analysis, Protozoan Proteins/chemistry, Rabbits, Reticulocytes/parasitology
Pubmed
Web of science
Open Access
Yes
Create date
22/04/2013 8:28
Last modification date
20/08/2019 14:46
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