Requirement of glucose metabolism for regulation of glucose transporter type 2 (GLUT2) gene expression in liver.

Details

Serval ID
serval:BIB_876599980A2C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Requirement of glucose metabolism for regulation of glucose transporter type 2 (GLUT2) gene expression in liver.
Journal
Biochemical Journal
Author(s)
Rencurel F., Waeber G., Antoine B., Rocchiccioli F., Maulard P., Girard J., Leturque A.
ISSN
0264-6021
Publication state
Published
Issued date
1996
Peer-reviewed
Oui
Volume
314
Number
3
Pages
903-909
Language
english
Abstract
Previous studies have shown that glucose increases the glucose transporter (GLUT2) mRNA expression in the liver in vivo and in vitro. Here we report an analysis of the effects of glucose metabolism on GLUT2 gene expression. GLUT2 mRNA accumulation by glucose was not due to stabilization of its transcript but rather was a direct effect on gene transcription. A proximal fragment of the 5' regulatory region of the mouse GLUT2 gene linked to a reporter gene was transiently transfected into liver GLUT2-expressing cells. Glucose stimulated reporter gene expression in these cells, suggesting that glucose-responsive elements were included within the proximal region of the promoter. A dose-dependent effect of glucose on GLUT2 expression was observed over 10 mM glucose irrespective of the hexokinase isozyme (glucokinase K(m) 16 mM; hexokinase I K(m) 0.01 mM) present in the cell type used. This suggests that the correlation between extracellular glucose and GLUT2 mRNA concentrations is simply a reflection of an activation of glucose metabolism. The mediators and the mechanism responsible for this response remain to be determined. In conclusion, glucose metabolism is required for the proper induction of the GLUT2 gene in the liver and this effect is transcriptionally regulated.
Keywords
Acetylglucosamine, Amanitins, Animals, Cells, Cultured, Dactinomycin, Female, Gene Expression Regulation, Glucose, Glucose Transporter Type 2, Kinetics, Liver, Mice, Monosaccharide Transport Proteins, Protein Synthesis Inhibitors, Puromycin, RNA, Messenger, Rats, Rats, Wistar, Recombinant Proteins, Regulatory Sequences, Nucleic Acid, Transcription, Genetic, Transfection
Pubmed
Web of science
Create date
25/01/2008 14:10
Last modification date
20/08/2019 14:46
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