Isolation of two closely related vitellogenin genes, including their flanking regions, from a Xenopus laevis gene library.
Details
Serval ID
serval:BIB_86FADFB4E6A7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Isolation of two closely related vitellogenin genes, including their flanking regions, from a Xenopus laevis gene library.
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424[print], 0027-8424[linking]
Publication state
Published
Issued date
03/1980
Volume
77
Number
3
Pages
1437-1441
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
A gene library of Xenopus laevis was constructed from embryonic DNA partially digested with restriction endonucleases Hae III and Alu I and joined to the phage lambda Charon 4 cloning vector with EcoRI linkers. Nucleotide sequences from three of the four related vitellogenin genes have been isolated. Two of the genes (called A1 and A2) were isolated in their entirety together with long stretches of flanking sequences. These two closely related vitellogenin genes have lengths of about 21 and 16 kilobases, but both produce a vitellogenin mRNA of 6.3 kilobases.
Keywords
Animals, Bacteriophage lambda/genetics, Base Sequence, Cloning, Molecular/methods, Genes, Lipoproteins/genetics, Molecular Weight, Nucleic Acid Hybridization, Nucleic Acid Precursors/genetics, RNA, Messenger/genetics, Vitellogenins/genetics, Xenopus/genetics
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 17:04
Last modification date
20/08/2019 15:46
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