Processing of the Pseudomonas arcDABC mRNA requires functional RNase E in Escherichia coli.

Details

Serval ID
serval:BIB_86159552DD72
Type
Article: article from journal or magazin.
Collection
Publications
Title
Processing of the Pseudomonas arcDABC mRNA requires functional RNase E in Escherichia coli.
Journal
Gene
Author(s)
Gamper M., Haas D.
ISSN
0378-1119 (Print)
ISSN-L
0378-1119
Publication state
Published
Issued date
1993
Volume
129
Number
1
Pages
119-122
Language
english
Abstract
In Pseudomonas aeruginosa, arginine catabolism via the arginine deiminase pathway depends on the anaerobically inducible arcDABC operon, whose expression is further modulated by mRNA processing. Fusion of the cloned arc operon to an external tac promoter did not alter the processing pattern in P. aeruginosa and allowed heterologous expression in Escherichia coli. Processing within a specific region of the arcD mRNA was similar in P. aeruginosa and in E. coli. In E. coli, a conditional temperature-sensitive (ts) mutation in the gene specifying RNase E prevented cleavage of the arc mRNA at the non-permissive temperature, whereas mutations in the genes encoding RNase III or RNase P had no effect. We therefore speculate that in P. aeruginosa, an RNase E-like enzyme exists which is involved in the specific processing of the arc mRNA.
Keywords
Amino Acid Transport Systems, Antiporters, Arginine/metabolism, Bacterial Proteins/genetics, Endoribonucleases/metabolism, Escherichia coli/enzymology, Gene Expression Regulation, Bacterial, Membrane Proteins/genetics, Operon, Pseudomonas aeruginosa/genetics, RNA Processing, Post-Transcriptional, RNA, Bacterial/metabolism, RNA, Messenger/genetics, RNA, Messenger/metabolism
Pubmed
Web of science
Create date
25/01/2008 17:00
Last modification date
20/08/2019 14:45
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