Processing of the Pseudomonas arcDABC mRNA requires functional RNase E in Escherichia coli.

Détails

ID Serval
serval:BIB_86159552DD72
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Processing of the Pseudomonas arcDABC mRNA requires functional RNase E in Escherichia coli.
Périodique
Gene
Auteur(s)
Gamper M., Haas D.
ISSN
0378-1119 (Print)
ISSN-L
0378-1119
Statut éditorial
Publié
Date de publication
1993
Volume
129
Numéro
1
Pages
119-122
Langue
anglais
Résumé
In Pseudomonas aeruginosa, arginine catabolism via the arginine deiminase pathway depends on the anaerobically inducible arcDABC operon, whose expression is further modulated by mRNA processing. Fusion of the cloned arc operon to an external tac promoter did not alter the processing pattern in P. aeruginosa and allowed heterologous expression in Escherichia coli. Processing within a specific region of the arcD mRNA was similar in P. aeruginosa and in E. coli. In E. coli, a conditional temperature-sensitive (ts) mutation in the gene specifying RNase E prevented cleavage of the arc mRNA at the non-permissive temperature, whereas mutations in the genes encoding RNase III or RNase P had no effect. We therefore speculate that in P. aeruginosa, an RNase E-like enzyme exists which is involved in the specific processing of the arc mRNA.
Mots-clé
Amino Acid Transport Systems, Antiporters, Arginine/metabolism, Bacterial Proteins/genetics, Endoribonucleases/metabolism, Escherichia coli/enzymology, Gene Expression Regulation, Bacterial, Membrane Proteins/genetics, Operon, Pseudomonas aeruginosa/genetics, RNA Processing, Post-Transcriptional, RNA, Bacterial/metabolism, RNA, Messenger/genetics, RNA, Messenger/metabolism
Pubmed
Web of science
Création de la notice
25/01/2008 17:00
Dernière modification de la notice
20/08/2019 14:45
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