Nanopore detection of single molecule RNAP-DNA transcription complex.

Details

Serval ID
serval:BIB_846A9ACCE57B
Type
Article: article from journal or magazin.
Publication sub-type
Letter (letter): Communication to the publisher.
Collection
Publications
Title
Nanopore detection of single molecule RNAP-DNA transcription complex.
Journal
Nano Letters
Author(s)
Raillon C., Cousin P., Traversi F., Garcia-Cordero E., Hernandez N., Radenovic A.
ISSN
1530-6992 (Electronic)
ISSN-L
1530-6984
Publication state
Published
Issued date
2012
Volume
12
Number
3
Pages
1157-1164
Language
english
Abstract
In the past decade, a number of single-molecule methods have been developed with the aim of investigating single protein and nucleic acid interactions. For the first time we use solid-state nanopore sensing to detect a single E. coli RNAP-DNA transcription complex and single E. coli RNAP enzyme. On the basis of their specific conductance translocation signature, we can discriminate and identify between those two types of molecular translocations and translocations of bare DNA. This opens up a new perspectives for investigating transcription processes at the single-molecule level.
Pubmed
Web of science
Create date
05/05/2012 16:55
Last modification date
20/08/2019 15:44
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