Gene profiling analysis of the early effects of ablative fractional carbon dioxide laser treatment on human skin.

Details

Serval ID
serval:BIB_837CBC1D9B50
Type
Article: article from journal or magazin.
Collection
Publications
Title
Gene profiling analysis of the early effects of ablative fractional carbon dioxide laser treatment on human skin.
Journal
Dermatologic Surgery
Author(s)
Kim J.E., Won C.H., Bak H., Kositratna G., Manstein D., Dotto G.P., Chang S.E.
ISSN
1524-4725 (Electronic)
ISSN-L
1076-0512
Publication state
Published
Issued date
2013
Peer-reviewed
Oui
Volume
39
Number
7
Pages
1033-1043
Language
english
Abstract
BACKGROUND: The use of carbon dioxide (CO2) laser-mediated ablative fractional resurfacing (AFR) is currently under extensive clinical investigation, but the molecular mechanisms underlying this process are unclear.
OBJECTIVES: To determine the early expressed genes that are upregulated in human skin after treatment using a CO2 fractional laser.
METHODS: Whole human skin was irradiated using an AFR CO2 laser, and changes in gene expression after 2 and 24 hours were analyzed using microarray analysis. The results were validated using reverse transcriptase polymerase chain reaction (RT-PCR). Laser scanning confocal microscopy (LSCM) was used to investigate the expression of the validated proteins after AFR CO2 laser treatment of skin that had been biopsied from seven Korean patients.
RESULTS: Gene expression profiling showed that the most significantly upregulated genes in these skin samples were those encoding Wnt5a, cysteine-rich angiogenic inducer 61 (CYR61), and heat shock protein (HSP) 90. These results were confirmed using real-time RT-PCR and LSCM.
CONCLUSIONS: Irradiation using an AFR laser may induce the expression of Wnt5a, CYR61, and HSP90 in human skin during the early remodeling phases, suggesting that the induction of proteins may be the preceding event that is associated with the clinical effects of laser treatment.
Keywords
Carbon Dioxide, Collagen/metabolism, Cysteine-Rich Protein 61/metabolism, Dermabrasion, HSP90 Heat-Shock Proteins/metabolism, Humans, Laser Therapy/methods, Matrix Metalloproteinases/metabolism, Microscopy, Confocal, Proto-Oncogene Proteins/metabolism, Skin/radiation effects, Tissue Array Analysis, Up-Regulation/physiology, Wnt Proteins/metabolism, Wound Healing/physiology
Pubmed
Create date
24/09/2014 8:25
Last modification date
20/08/2019 14:43
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