Sce3, a suppressor of the Schizosaccharomyces pombe septation mutant cdc11, encodes a putative RNA-binding protein.

Details

Serval ID
serval:BIB_837
Type
Article: article from journal or magazin.
Collection
Publications
Title
Sce3, a suppressor of the Schizosaccharomyces pombe septation mutant cdc11, encodes a putative RNA-binding protein.
Journal
Nucleic Acids Research
Author(s)
Schmidt S., Hofmann K., Simanis V.
ISSN
0305-1048
Publication state
Published
Issued date
1997
Volume
25
Number
17
Pages
3433-3439
Language
english
Notes
Publication types: Comparative Study ; Journal Article
Abstract
In the fission yeast Schizosaccharomyces pombe, the cdc11 gene is required for the initiation of septum formation at the end of mitosis. The sce3 gene was cloned as a multi-copy suppressor of the heat-sensitive mutant cdc11-136. When over-expressed, it rescues all mutants of cdc11 and also a heat-sensitive allele of cdc14, but not the cdc14 null mutant. Deletion shows that sce3 is not essential for cell proliferation. It encodes a putative RNA-binding protein which shows homology to human eIF4B. Immunolocalisation indicates that Sce3p is located predominantly in the cytoplasm. Elevated expression of sce3 increases the steady-state level of cdc14 mRNA. Possible mechanisms of its action are discussed.
Keywords
Amino Acid Sequence, Cell Cycle/genetics, Cell Cycle Proteins/genetics, Cell Division, Cloning, Molecular, Cytoskeletal Proteins, Eukaryotic Initiation Factors, Fungal Proteins/genetics, Gene Deletion, Genes, Fungal, Genes, Suppressor, Humans, Molecular Sequence Data, Mutation, Peptide Initiation Factors/chemistry, Protein Tyrosine Phosphatases, RNA, Messenger/metabolism, RNA-Binding Proteins/chemistry, RNA-Binding Proteins/genetics, Saccharomyces cerevisiae Proteins, Schizosaccharomyces/genetics, Schizosaccharomyces/physiology, Schizosaccharomyces pombe Proteins, Sequence Alignment
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 13:46
Last modification date
08/05/2019 21:16
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