Article: article from journal or magazin.
Sce3, a suppressor of the Schizosaccharomyces pombe septation mutant cdc11, encodes a putative RNA-binding protein.
Nucleic Acids Research
Publication types: Comparative Study ; Journal Article
In the fission yeast Schizosaccharomyces pombe, the cdc11 gene is required for the initiation of septum formation at the end of mitosis. The sce3 gene was cloned as a multi-copy suppressor of the heat-sensitive mutant cdc11-136. When over-expressed, it rescues all mutants of cdc11 and also a heat-sensitive allele of cdc14, but not the cdc14 null mutant. Deletion shows that sce3 is not essential for cell proliferation. It encodes a putative RNA-binding protein which shows homology to human eIF4B. Immunolocalisation indicates that Sce3p is located predominantly in the cytoplasm. Elevated expression of sce3 increases the steady-state level of cdc14 mRNA. Possible mechanisms of its action are discussed.
Amino Acid Sequence, Cell Cycle/genetics, Cell Cycle Proteins/genetics, Cell Division, Cloning, Molecular, Cytoskeletal Proteins, Eukaryotic Initiation Factors, Fungal Proteins/genetics, Gene Deletion, Genes, Fungal, Genes, Suppressor, Humans, Molecular Sequence Data, Mutation, Peptide Initiation Factors/chemistry, Protein Tyrosine Phosphatases, RNA, Messenger/metabolism, RNA-Binding Proteins/chemistry, RNA-Binding Proteins/genetics, Saccharomyces cerevisiae Proteins, Schizosaccharomyces/genetics, Schizosaccharomyces/physiology, Schizosaccharomyces pombe Proteins, Sequence Alignment
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