Nasal resistome development in infants with cystic fibrosis in the first year of life

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_7EA36E4E2B15
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Nasal resistome development in infants with cystic fibrosis in the first year of life
Journal
Frontiers in Microbiology
Author(s)
Allemann A., Kraemer J.G., Korten I., Ramsey K., Casaulta C., Wüthrich D., Ramette A., Endimiani A., Latzin P., Hilty M.
Working group(s)
SCILD Study Group
ISSN
1664-302X (Print)
ISSN-L
1664-302X
Publication state
Published
Issued date
2019
Peer-reviewed
Oui
Volume
10
Number
212
Pages
[1-14]
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Polymicrobial infections of the respiratory tract due to antibiotic resistant bacteria are a great concern in patients with cystic fibrosis (CF). We therefore aimed at establishing a functional metagenomic method to analyze the nasal resistome in infants with CF within the first year of life. We included samples from patients before antibiotic treatment, which allowed obtaining information regarding natural status of the resistome. In total, we analyzed 130 nasal swabs from 26 infants with CF and screened for β-lactams (ampicillin, amoxicillin-clavulanic acid, and cefuroxime) and other classes of antibiotic resistances (tetracycline, chloramphenicol and trimethoprim-sulfamethoxazole). For 69 swabs (53% of total), we found at least one non-susceptible phenotype. Analyses of the inserts recovered from non-susceptible clones by nanopore MinION sequencing revealed a large reservoir of resistance genes including mobile elements within the antibiotic naïve samples. Comparing the data of the resistome with the microbiota composition showed that the bacterial phyla and operational taxonomic units (OTUs) of the microbiota rather than the antibiotic treatment were associated with the majority of non-susceptible phenotypes in the resistome. Future studies will reveal if characterization of the resistome can help in clinical decision-making in patients with CF.
Keywords
antibiotics, beta-lactamases, functional metagenomics, nasal swabs, resistance
Pubmed
Web of science
Open Access
Yes
Create date
08/04/2019 16:23
Last modification date
20/08/2019 14:39
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