Cysteine: depolarization-induced release from rat brain in vitro.

Details

Serval ID
serval:BIB_7E2F0A0B2F8E
Type
Article: article from journal or magazin.
Collection
Publications
Title
Cysteine: depolarization-induced release from rat brain in vitro.
Journal
Journal of Neurochemistry
Author(s)
Keller H.J., Do K.Q., Zollinger M., Winterhalter K.H., Cuénod M.
ISSN
0022-3042 (Print)
ISSN-L
0022-3042
Publication state
Published
Issued date
1989
Peer-reviewed
Oui
Volume
52
Number
6
Pages
1801-1806
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
Compounds released on depolarization in a Ca2+-dependent manner from rat brain slices were screened to identify candidates for neuroactive substances. Lyophilized superfusates were analyzed by reversed-phase HPLC after derivatization with 9-fluorenyl N-succinimidyl carbonate. One of the compounds that showed an increase of concentration in superfusates in the presence of iodoacetamide was identified as the cysteine (Cys) derivative, S-carboxamidomethylcysteine, by fast atom bombardment mass spectrometry and other methods. This stable Cys derivative originates from endogenous, extracellular Cys. The finding led to a method for quantification of Cys in superfusates by immediate cooling of the superfusates to 0 degrees C and reaction of Cys with N-ethylmaleimide. Depolarization-induced Ca2+-dependent release of Cys was most prominent in the neocortex, followed by the mesodiencephalon, striatum, and cerebellum. This suggests that Cys is released from a neuronal compartment and might be involved in neurotransmission.
Keywords
Animals, Brain/metabolism, Brain/physiology, Chromatography, High Pressure Liquid, Cysteine/analysis, Cysteine/metabolism, Electrophysiology, Male, Rats, Rats, Inbred Strains
Pubmed
Web of science
Create date
06/03/2014 16:26
Last modification date
20/08/2019 14:39
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