Dual microsporidial infection due to Vittaforma corneae and Encephalitozoon hellem in a patient with AIDS.
Details
State: Public
Version: Final published version
License: Not specified
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
Serval ID
serval:BIB_7972
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Dual microsporidial infection due to Vittaforma corneae and Encephalitozoon hellem in a patient with AIDS.
Journal
Clinical Infectious Diseases
ISSN
1058-4838 (Print)
ISSN-L
1058-4838
Publication state
Published
Issued date
1998
Volume
27
Number
6
Pages
1521-1524
Language
english
Notes
Publication types: Case Reports ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
A 46-year-old human immunodeficiency virus-infected Swiss citizen living in Tanzania presented with respiratory, abdominal, and urogenital complaints. Microsporidial spores were isolated from urine and a sinunasal aspirate and were propagated in MRC-5 cell cultures. Western blot analysis and riboprinting identified the sinunasal isolate as Encephalitozoon hellem. Electron microscopic investigation of the urine isolate revealed spores with diplokaryotic nuclei and five to six isofilar coils of the polar tube and sporonts with two or three diplokarya. All stages were enveloped by two membranes, corresponding to a cisterna of host endoplasmic reticulum studded with ribosomes. These characteristics have been described for the genus Vittaforma. Western blot analysis of this isolate revealed a banding pattern identical to that of the Vittaforma corneae reference isolate. Part of the small subunit rRNA gene was amplified, sequenced (239 base pairs), and found to be identical to that of V. corneae. This is the second isolation of V. corneae and the first description of urinary tract infection due to V. corneae in a patient with AIDS.
Keywords
AIDS-Related Opportunistic Infections/parasitology, Animals, Blotting, Western, Encephalitozoon/isolation & purification, Encephalitozoonosis/complications, Humans, Male, Mice, Mice, Nude/parasitology, Microsporida/isolation & purification, Microsporida/ultrastructure, Middle Aged, Protozoan Infections/parasitology
OAI-PMH
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 13:46
Last modification date
14/02/2022 8:55