The SM protein Vps33 and the t-SNARE H(abc) domain promote fusion pore opening.

Details

Serval ID
serval:BIB_784AB373153A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The SM protein Vps33 and the t-SNARE H(abc) domain promote fusion pore opening.
Journal
Nature Structural and Molecular Biology
Author(s)
Pieren M., Schmidt A., Mayer A.
ISSN
1545-9985[electronic], 1545-9985[linking]
Publication state
Published
Issued date
2010
Volume
17
Number
6
Pages
710-717
Language
english
Abstract
Intracellular membrane fusion proceeds via distinct stages of membrane docking, hemifusion and fusion pore opening and depends on interacting families of Rab, SNARE and SM proteins. Trans-SNARE complexes dock the membranes in close apposition. Efficient fusion requires further SNARE-associated proteins. They might increase the number of trans-SNARE complexes or the fusogenic potential of a single SNARE complex. We investigated the contributions of the SM protein Vps33 to hemifusion and pore opening between yeast vacuoles. Mutations in Vps33 that weaken its interactions with the SNARE complex allowed normal trans-SNARE pairing and lipid mixing but retarded content mixing. Deleting the H(abc) domain of the vacuolar t-SNARE Vam3, which interacts with Vps33, had the same effect. This suggests that SM proteins promote fusion pore opening by enhancing the fusogenic activity of a SNARE complex. They should thus be considered integral parts of the fusion machinery.
Keywords
Amino Acid Sequence, Base Sequence, DNA Primers/genetics, Membrane Fusion/genetics, Membrane Fusion/physiology, Models, Molecular, Molecular Sequence Data, Multiprotein Complexes, Mutation, Protein Interaction Domains and Motifs, Qa-SNARE Proteins/chemistry, Qa-SNARE Proteins/genetics, Recombinant Proteins/chemistry, Recombinant Proteins/genetics, SNARE Proteins/chemistry, SNARE Proteins/genetics, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae/metabolism, Saccharomyces cerevisiae Proteins/chemistry, Saccharomyces cerevisiae Proteins/genetics, Sequence Homology, Amino Acid, Vacuoles/metabolism, Vesicular Transport Proteins/chemistry, Vesicular Transport Proteins/genetics
Pubmed
Web of science
Create date
24/09/2010 8:01
Last modification date
20/08/2019 14:35
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