The SM protein Vps33 and the t-SNARE H(abc) domain promote fusion pore opening.

Détails

ID Serval
serval:BIB_784AB373153A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The SM protein Vps33 and the t-SNARE H(abc) domain promote fusion pore opening.
Périodique
Nature Structural and Molecular Biology
Auteur(s)
Pieren M., Schmidt A., Mayer A.
ISSN
1545-9985[electronic], 1545-9985[linking]
Statut éditorial
Publié
Date de publication
2010
Volume
17
Numéro
6
Pages
710-717
Langue
anglais
Résumé
Intracellular membrane fusion proceeds via distinct stages of membrane docking, hemifusion and fusion pore opening and depends on interacting families of Rab, SNARE and SM proteins. Trans-SNARE complexes dock the membranes in close apposition. Efficient fusion requires further SNARE-associated proteins. They might increase the number of trans-SNARE complexes or the fusogenic potential of a single SNARE complex. We investigated the contributions of the SM protein Vps33 to hemifusion and pore opening between yeast vacuoles. Mutations in Vps33 that weaken its interactions with the SNARE complex allowed normal trans-SNARE pairing and lipid mixing but retarded content mixing. Deleting the H(abc) domain of the vacuolar t-SNARE Vam3, which interacts with Vps33, had the same effect. This suggests that SM proteins promote fusion pore opening by enhancing the fusogenic activity of a SNARE complex. They should thus be considered integral parts of the fusion machinery.
Mots-clé
Amino Acid Sequence, Base Sequence, DNA Primers/genetics, Membrane Fusion/genetics, Membrane Fusion/physiology, Models, Molecular, Molecular Sequence Data, Multiprotein Complexes, Mutation, Protein Interaction Domains and Motifs, Qa-SNARE Proteins/chemistry, Qa-SNARE Proteins/genetics, Recombinant Proteins/chemistry, Recombinant Proteins/genetics, SNARE Proteins/chemistry, SNARE Proteins/genetics, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae/metabolism, Saccharomyces cerevisiae Proteins/chemistry, Saccharomyces cerevisiae Proteins/genetics, Sequence Homology, Amino Acid, Vacuoles/metabolism, Vesicular Transport Proteins/chemistry, Vesicular Transport Proteins/genetics
Pubmed
Web of science
Création de la notice
24/09/2010 8:01
Dernière modification de la notice
20/08/2019 14:35
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