Disrupted NF- kappa B activation after partial hepatectomy does not impair hepatocyte proliferation in rats.

Details

Serval ID
serval:BIB_77E6B679FF32
Type
Article: article from journal or magazin.
Collection
Publications
Title
Disrupted NF- kappa B activation after partial hepatectomy does not impair hepatocyte proliferation in rats.
Journal
World Journal of Gastroenterology
Author(s)
Laurent S., Horsmans Y., Stärkel P., Leclercq I., Sempoux C., Lambotte L.
ISSN
1007-9327 (Print)
ISSN-L
1007-9327
Publication state
Published
Issued date
2005
Peer-reviewed
Oui
Volume
11
Number
46
Pages
7345-7350
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
AIM: To analyze the effects of NF- kappa B inhibition by antioxidant pyrrolidine dithiocarbamate (PDTC) or TNF inhibitor pentoxifylline (PTX) on liver regeneration after partial hepatectomy (PH).
METHODS: Saline, PDTC or PTX were injected 1 h before PH and rats were killed at 0.5 and 24 h after PH. Several control groups were used for comparison (injection control groups).
RESULTS: Compared to saline injected controls, NF- kappa B activation was absent 0.5 h after PH in rats treated with PDTC or PTX. At 24 h after PH, DNA synthesis and PCNA expression were identical in treated and control rats and thus occurred irrespectively of the status of NF- kappa B activation at 0.5 h. Signal transducer and activator of transcription 3 (Stat3) activation was observed already 0.5 h after PH in saline, PDTC or PTX group and was similar to Stat3 activation in response to injection without PH.
CONCLUSION: These data strongly suggest that (1) NF- kappa B p65/p50 DNA binding produced in response to PH is not a signal necessary to initiate the liver regeneration, (2) Stat3 activation is a stress response unrelated to the activation of NF- kappa B. In conclusion, NF- kappa B activation is not critically required for the process of liver regeneration after PH.
Keywords
Animals, Cell Proliferation/drug effects, Hepatectomy, Hepatocytes/cytology, Hepatocytes/drug effects, Liver Regeneration/drug effects, Liver Regeneration/physiology, Male, NF-kappa B/antagonists & inhibitors, NF-kappa B/metabolism, Pentoxifylline/pharmacology, Pyrrolidines/pharmacology, Rats, Rats, Wistar, STAT3 Transcription Factor/metabolism, Thiocarbamates/pharmacology, Tumor Necrosis Factor-alpha/antagonists & inhibitors
Pubmed
Web of science
Create date
20/10/2016 16:15
Last modification date
20/08/2019 14:34
Usage data