Disrupted NF- kappa B activation after partial hepatectomy does not impair hepatocyte proliferation in rats.
Details
Serval ID
serval:BIB_77E6B679FF32
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Disrupted NF- kappa B activation after partial hepatectomy does not impair hepatocyte proliferation in rats.
Journal
World Journal of Gastroenterology
ISSN
1007-9327 (Print)
ISSN-L
1007-9327
Publication state
Published
Issued date
2005
Peer-reviewed
Oui
Volume
11
Number
46
Pages
7345-7350
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
AIM: To analyze the effects of NF- kappa B inhibition by antioxidant pyrrolidine dithiocarbamate (PDTC) or TNF inhibitor pentoxifylline (PTX) on liver regeneration after partial hepatectomy (PH).
METHODS: Saline, PDTC or PTX were injected 1 h before PH and rats were killed at 0.5 and 24 h after PH. Several control groups were used for comparison (injection control groups).
RESULTS: Compared to saline injected controls, NF- kappa B activation was absent 0.5 h after PH in rats treated with PDTC or PTX. At 24 h after PH, DNA synthesis and PCNA expression were identical in treated and control rats and thus occurred irrespectively of the status of NF- kappa B activation at 0.5 h. Signal transducer and activator of transcription 3 (Stat3) activation was observed already 0.5 h after PH in saline, PDTC or PTX group and was similar to Stat3 activation in response to injection without PH.
CONCLUSION: These data strongly suggest that (1) NF- kappa B p65/p50 DNA binding produced in response to PH is not a signal necessary to initiate the liver regeneration, (2) Stat3 activation is a stress response unrelated to the activation of NF- kappa B. In conclusion, NF- kappa B activation is not critically required for the process of liver regeneration after PH.
METHODS: Saline, PDTC or PTX were injected 1 h before PH and rats were killed at 0.5 and 24 h after PH. Several control groups were used for comparison (injection control groups).
RESULTS: Compared to saline injected controls, NF- kappa B activation was absent 0.5 h after PH in rats treated with PDTC or PTX. At 24 h after PH, DNA synthesis and PCNA expression were identical in treated and control rats and thus occurred irrespectively of the status of NF- kappa B activation at 0.5 h. Signal transducer and activator of transcription 3 (Stat3) activation was observed already 0.5 h after PH in saline, PDTC or PTX group and was similar to Stat3 activation in response to injection without PH.
CONCLUSION: These data strongly suggest that (1) NF- kappa B p65/p50 DNA binding produced in response to PH is not a signal necessary to initiate the liver regeneration, (2) Stat3 activation is a stress response unrelated to the activation of NF- kappa B. In conclusion, NF- kappa B activation is not critically required for the process of liver regeneration after PH.
Keywords
Animals, Cell Proliferation/drug effects, Hepatectomy, Hepatocytes/cytology, Hepatocytes/drug effects, Liver Regeneration/drug effects, Liver Regeneration/physiology, Male, NF-kappa B/antagonists & inhibitors, NF-kappa B/metabolism, Pentoxifylline/pharmacology, Pyrrolidines/pharmacology, Rats, Rats, Wistar, STAT3 Transcription Factor/metabolism, Thiocarbamates/pharmacology, Tumor Necrosis Factor-alpha/antagonists & inhibitors
Pubmed
Web of science
Create date
20/10/2016 17:15
Last modification date
20/08/2019 15:34
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