Welche Funktionen haben sechs verschiedene Gene fur sekretorische Proteinasen von Candida albicans? [What functions do six different genes for secretory proteinases have in Candida albicans?]

Details

Serval ID
serval:BIB_77BEC5DA6BDB
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Welche Funktionen haben sechs verschiedene Gene fur sekretorische Proteinasen von Candida albicans? [What functions do six different genes for secretory proteinases have in Candida albicans?]
Journal
Mycoses
Author(s)
Hube  B., Sanglard  D., Schaller  M., Ibrahim  A., Odds  F. C., Gow  N. A.
ISSN
0933-7407 (Print)
Publication state
Published
Issued date
1998
Volume
41 Suppl 1
Pages
47-50
Notes
English Abstract
Journal Article
Review
Abstract
Secreted Aspartate Proteinases (Sap) are among those factors of the human pathogen Candida albicans, which promote infections in the immunocompromised host. Sap isoenzymes are encoded by at least nine different genes (SAP1-9), which are differentially regulated in vitro. RT-PCR analysis during experimental infections and from patient samples confirmed the expression of SAP genes in vivo. However, while Sap2 is the dominant isoenzyme under culture conditions, other SAP genes are also expressed during infections. In order to investigate the role of single isoenzymes during the pathogenesis of candidosis, mutants were produced which harbour deletions in SAP1, SAP2, SAP3 and SAP4-6. Although only SAP2 and SAP4-6 mutants showed a strong reduction of proteolytic activity in vitro, all SAP mutants were significantly attenuated in systemic infections. In addition, SAP2, SAP3 and SAP4-6 mutants were clearly more sensitive to neutrophilic leucocytes compared to the wild type SC5314. These investigations show that several proteinase isoenzymes are likely to be involved in the pathogenesis of candidosis.
Keywords
Candida albicans/*enzymology/*genetics/pathogenicity Candidiasis/enzymology/*etiology/genetics Endopeptidases/*genetics/physiology/secretion *Gene Expression Regulation, Fungal Humans Hydrolysis Isoenzymes/*genetics/physiology/secretion Membrane Proteins/metabolism Multigene Family Mutation Polymerase Chain Reaction Virulence
Pubmed
Web of science
Create date
25/01/2008 15:40
Last modification date
20/08/2019 15:34
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