Micro-patterning of biomolecules on polymer substrates.

Details

Serval ID
serval:BIB_76E6DB210730
Type
Article: article from journal or magazin.
Collection
Publications
Title
Micro-patterning of biomolecules on polymer substrates.
Journal
Langmuir
Author(s)
Schwarz A., Rossier J.S., Roulet E., Mermod N., Roberts M.A., Girault H.H.
ISSN
0743-7463[print], 1520-5827[electronic]
Publication state
Published
Issued date
1998
Volume
14
Number
19
Pages
5526-5531
Language
english
Abstract
UV−excimer laser photoablation was used, in combination with surface blocking techniques, to pattern proteins on the surfaces of polyimide and poly(ethylene terephthalate). This technique involves physical adsorption of avidin through laser-defined openings in low-temperature laminates or adsorbed protein blocking layers. Visualization of biomolecular patterns were monitored using avidin and fluorescein-labeled biotin as a model receptor−ligand couple. Adsorbed proteins could be shown to bind to UV-laser-treated polymer surfaces up to three times higher than on commercially available polymers. UV-laser photoablation was also used for the generation of three-dimensional structure, which leads to the possibility of biomolecule patterning within polymer-based microanalytical systems. The simplicity and easy handling of the described technique facilitate its application in microdiagnostic devices.
Web of science
Create date
20/03/2008 14:04
Last modification date
20/08/2019 15:34
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