High tumour contamination of leukaphereses in patients with small cell carcinoma of the lung: a comparison of immunocytochemistry and RT-PCR
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State: Public
Version: Final published version
State: Public
Version: Final published version
Serval ID
serval:BIB_75042F3A3F45
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
High tumour contamination of leukaphereses in patients with small cell carcinoma of the lung: a comparison of immunocytochemistry and RT-PCR
Journal
British Journal of Cancer
ISSN
0007-0920 (Print)
Publication state
Published
Issued date
11/2001
Volume
85
Number
11
Pages
1713-21
Language
english
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov 30
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov 30
Abstract
In small-cell lung carcinoma (SCLC) tumour cell contamination of leukaphereses is unknown. The present study was performed to define appropriate markers for reverse transcriptase polymerase chain reaction (RT-PCR), then to assess the contamination rate of leukaphereses and corresponding bone marrow samples. Immunocytochemistry (ICC) and RT-PCR methods were also compared. Among the 33 patients included, analyses were performed in 16 who had multiple leukaphereses and 17 who had only bone marrow. Leukapheresis products and bone marrow were analysed by ICC using several specific monoclonal antibodies against neural-cell adhesion molecule (N-CAM), epithelial glycoprotein (EGP-40) and cytokeratins (CK). Samples were also analyzed by RT-PCR for expression for N-CAM, synaptophysin, neuron-specific enolase, chromogranin, cytokeratin-18/-19, CEA, EGP-40, apomucin type 1 (MUC-1) and human endothelial cell-specific molecule (ESM-1). Using ICC staining, contaminating tumour cells were detected in 34% of leukaphereses (27% in patients with limited disease and 43% in those with extensive disease). N-CAM was the most reliable marker for detection of contamination. For RT-PCR, CK-19 and CEA were the only appropriate markers. Positive signal rate in leukaphereses increased to 78% (89% for patients with limited disease and 67% for extensive disease). In bone marrow, both techniques were in agreement whereas in leukaphereses, RT-PCR was better than ICC. A high rate of tumour cell contamination was demonstrated not only in bone marrow but also in leukaphereses from SCLC patients. The most appropriate technique was RT-PCR mainly in patients with limited disease.
Keywords
Adult
Aged
Antigens, Neoplasm/analysis/genetics
Bone Marrow/chemistry/pathology
Carcinoembryonic Antigen/analysis/genetics
Carcinoma, Small Cell/blood/*therapy
Cell Adhesion Molecules/analysis/genetics
Chromogranins/analysis/genetics
Female
Gastric Mucin/analysis/genetics
Humans
Immunohistochemistry
Keratins/analysis/genetics
*Leukapheresis
Lung Neoplasms/blood/*therapy
Male
Middle Aged
Neoplasm Circulating Cells/metabolism/*pathology
*Neoplasm Proteins
Neural Cell Adhesion Molecules/analysis/genetics
Phosphopyruvate Hydratase/analysis/genetics
Proteins/analysis/genetics
*Proteoglycans
RNA, Neoplasm/genetics/metabolism
Reverse Transcriptase Polymerase Chain Reaction
Synaptophysin/analysis/genetics
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 9:31
Last modification date
20/08/2019 15:32