Interactions between NPY and its receptor: assessment using ant-NPY antibodies
Details
Serval ID
serval:BIB_7503C394A5F2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Interactions between NPY and its receptor: assessment using ant-NPY antibodies
Journal
Regulatory Peptides
ISSN
0167-0115 (Print)
Publication state
Published
Issued date
12/1994
Volume
54
Number
2-3
Pages
439-44
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec 15
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec 15
Abstract
The present data show that monoclonal antibodies (NPY02, NPY03, NPY04, NPY05) directed against 4 distinct epitopes on NPY may have different actions on NPY binding and NPY-induced cellular responses. NPY02 and NPY05 recognize the 11-24 and 32-36 amidated form of NPY, respectively. These 2 antibodies block the binding of NPY to its receptor as well as the NPY-induced inhibition of cAMP accumulation caused in SK-N-MC cells by forskolin. NPY02 and NPY05 have also an inhibitory action on NPY-induced contraction of rabbit femoral arteries. NPY03 and NPY04 are directed against the 27-34 and 1-12 part of NPY, respectively. NPY03 and NPY04 inhibit the binding of NPY only at very high concentrations and have a weak effect on cAMP response to NPY. NPY02 and NPY05 might provide useful tools to study the effect of NPY in cellular systems and organ preparations.
Keywords
Amino Acid Sequence
Antibodies, Monoclonal
Cyclic AMP/metabolism
Epitope Mapping
Humans
Immunologic Techniques
Molecular Sequence Data
Neuropeptide Y/*metabolism
Radioligand Assay
Receptors, Neuropeptide Y/*metabolism
Pubmed
Web of science
Create date
25/01/2008 11:55
Last modification date
20/08/2019 15:32
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