Apical and basolateral membrane conductances in the TBM cell line
Details
Serval ID
serval:BIB_7404A0EF91D8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Apical and basolateral membrane conductances in the TBM cell line
Journal
American Journal of Physiology
ISSN
0002-9513 (Print)
Publication state
Published
Issued date
06/1991
Volume
260
Number
6 Pt 1
Pages
C1172-81
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Research Support, Non-U.S. Gov't --- Old month value: Jun
Abstract
Cultured cell lines present several advantages over whole organ or ex vivo isolated epithelium for the physiological and biochemical study of epithelial transport. We have developed a new technique allowing for simultaneous intracellular and transepithelial electrophysiological measurements in the epithelium formed by a cultured cell line grown on thin collagen membranes. This technique was applied to the TBM 18/23 (toad bladder origin) cell line. The transepithelial and basolateral membrane potentials were -30 +/- 11 and -72 +/- 8 (SD) mV (n = 36), respectively. With the use of the effect of amiloride, which partially blocked the apical membrane conductance, and circuit analysis, the apical and basolateral membrane conductances were estimated to 0.7 +/- 0.1 and 2.8 +/- 0.4 mS/cm2, respectively. A sodium-selective conductive pathway was demonstrated in the apical membrane, and a barium-sensitive K(+)-selective conductance was shown to be present in the basolateral membrane. The basolateral membrane conductance was not modified by sudden inhibition of sodium transport by amiloride, but it was significantly reduced after a long-term decrease of Na+ transport. The cultured TBM cell line appears to be a convenient model to investigate the regulation of membrane ionic conductances in tight epithelia.
Keywords
Amiloride/pharmacology
Animals
Bufo marinus
Cell Line
Cell Membrane/drug effects/*physiology/ultrastructure
Collagen
Culture Techniques/instrumentation/methods
Electric Conductivity
Electrophysiology/instrumentation/methods
Epithelium/physiology
Kinetics
Mathematics
Membrane Potentials/drug effects
Microscopy, Electron
Urinary Bladder/physiology
Pubmed
Web of science
Create date
24/01/2008 13:37
Last modification date
20/08/2019 15:31