Human large granular lymphocytes contain an esterase activity usually considered as specific for the myeloid series.

Details

Serval ID
serval:BIB_72DB625A1DDE
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Human large granular lymphocytes contain an esterase activity usually considered as specific for the myeloid series.
Journal
European Journal of Immunology
Author(s)
Heumann D., Colombatti M., Mach J.P.
ISSN
0014-2980 (Print)
ISSN-L
0014-2980
Publication state
Published
Issued date
1983
Volume
13
Number
3
Pages
254-258
Language
english
Abstract
A cytochemical marker such as alpha-naphthyl acetate esterase (ANAE) has been found useful for the morphological identification of the subset of T lymphocytes having receptors for Fcμ (TM cells). ANAE reaction on TM cells gives a typical pattern of one to four positive spots, whereas this pattern is not found on T cells with receptors for Fcγ (TG cells). ANAE is abundant in monocytes but not detectable in granulocytes. Herein another type of esterase activity, naphthol-AS-D chloroacetate esterase (NCAE), is described; it is known to be abundant in granulocytes and was found to give a specific pattern of reactivity with the subpopulation of large granular lymphocytes (LGL). This pattern of fine granular staining was observed not only on LGL present in the TG cell subpopulation but also in LGL present in the non-T, non-B cells.
Fractions of peripheral blood mononuclear cells which were ènriched up to 80% in LGL by Percoll discontinuous density gradient gave a similar percentage of specific NCAE pattern. In addition, among the different fractions from Percoll gradient, there was a good correlation (r = 0.94) between the number of NCAE-positive cells and the natural killer activity against the natural killer susceptible K562 target cells. It will be important to determine whether or not this enzymatic activity plays a role in the cytotoxic activities of LGL.
Keywords
Adult, Carboxylic Ester Hydrolases/blood, Cell Line, Cytotoxicity, Immunologic, Humans, Killer Cells, Natural/immunology, Leukemia, Lymphoid/immunology, Lymphocytes/cytology, Lymphocytes/enzymology, Naphthol AS D Esterase/blood, Staining and Labeling, T-Lymphocytes/enzymology
Pubmed
Web of science
Create date
25/04/2013 14:49
Last modification date
20/08/2019 15:30
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