Technologies of directed protein evolution in vivo.
Details
State: Public
Version: Final published version
Serval ID
serval:BIB_72C4906655F8
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Technologies of directed protein evolution in vivo.
Journal
Cellular and Molecular Life Sciences
ISSN
1420-9071 (Electronic)
ISSN-L
1420-682X
Publication state
Published
Issued date
2011
Volume
68
Number
7
Pages
1207-1214
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Review
Abstract
Directed evolution of proteins for improved or modified functionality is an important branch of modern biotechnology. It has traditionally been performed using various in vitro methods, but more recently, methods of in vivo artificial evolution come into play. In this review, we discuss and compare prokaryotic and eukaryotic-based systems of directed protein evolution in vivo, highlighting their benefits and current limitations and focusing on the biotechnological potential of vertebrate immune cells for the generation of protein diversity by means of the immunoglobulin diversification machinery.
Keywords
Animals, Directed Molecular Evolution/methods, Eukaryota/genetics, Eukaryota/metabolism, Humans, Prokaryotic Cells/physiology, Protein Engineering/methods, Proteins/genetics
Pubmed
Create date
22/12/2011 16:10
Last modification date
20/08/2019 14:30
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