Costimulation provided by DNA immunization enhances antitumor immunity

Details

Serval ID
serval:BIB_7281EC9A3277
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Costimulation provided by DNA immunization enhances antitumor immunity
Journal
Journal of Immunology
Author(s)
Corr  M., Tighe  H., Lee  D., Dudler  J., Trieu  M., Brinson  D. C., Carson  D. A.
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
11/1997
Volume
159
Number
10
Pages
4999-5004
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Nov 15
Abstract
The interaction of the TCR with MHC class I-bound Ag is insufficient for the priming of CTL unless secondary costimulatory signals are provided. To ascertain the minimum elements required to activate an Ag-specific CTL response in vivo, we injected mice intradermally or i.m. with plasmid DNA encoding a MHC class I-restricted peptide Ag (minigene) and different membrane-bound costimulatory ligands. The minigene-encoded epitope only primed a specific CTL response if injected in the vicinity of an ectopically expressed costimulatory ligand. Vector encoding B7-1 was repeatedly more potent at stimulating a cytolytic response than vector encoding B7-2. In contrast the B7-2-encoding plasmid preferentially enhanced Ag-specific Ab responses when injected with either protein or a cDNA expression vector. Gene vaccination with plasmids encoding OVA and B7-1, but not B7-2, prolonged survival in mice challenged with an OVA-transfected tumor. These results show that functional B7-1 transfection can be achieved in vivo and induces the selective induction of CTL. The data suggest that B7-1 plasmids should be coadministered with naked DNA vaccines that aim to induce tumor-specific cellular immunity.
Keywords
Animals Antigens, CD/biosynthesis/physiology Antigens, CD86 DNA/*immunology Epitopes, T-Lymphocyte/genetics/immunology Female Histocompatibility Antigens Class I/genetics Immunoglobulin G/biosynthesis Ligands *Lymphocyte Activation/genetics Membrane Glycoproteins/biosynthesis/physiology Mice Mice, Inbred BALB C Mice, Inbred C57BL Neoplasm Transplantation Ovalbumin/immunology Plasmids/immunology Sarcoma, Experimental T-Lymphocytes, Cytotoxic/immunology Thymoma Tumor Cells, Cultured Tumor Escape/*genetics/immunology
Pubmed
Web of science
Create date
25/01/2008 8:31
Last modification date
20/08/2019 14:30
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