Humoral and cellular immune response to a Microsporum canis recombinant keratinolytic metalloprotease (r-MEP3) in experimentally infected guinea pigs
Details
Serval ID
serval:BIB_7271A7D5BE6D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Humoral and cellular immune response to a Microsporum canis recombinant keratinolytic metalloprotease (r-MEP3) in experimentally infected guinea pigs
Journal
Medical Mycology
ISSN
1369-3786 (Print)
Publication state
Published
Issued date
12/2003
Volume
41
Number
6
Pages
495-501
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Research Support, Non-U.S. Gov't --- Old month value: Dec
Abstract
In order to better understand the host-fungus relationship in Microsporum canis dermatophytosis and to identify major fungal antigens, the immune response to a crude exoantigen preparation and to a purified recombinant keratinolytic metalloprotease (r-MEP3) was evaluated in guinea pigs experimentally infected with M. canis. Humoral and cellular immune responses were assessed from day 0 to day 57 post-infection (PI), the former by enzyme-linked immunosorbent assay (ELISA) and the latter via a lymphocyte proliferation assay. Infected guinea pigs developed humoral and cellular responses to both M. canis exoantigen and r-MEP3, while no specific immune response to these antigens was observed in control animals. This is the first report on the development of both humoral and cell-mediated immune responses to a purified keratinase in M. canis dermatophytosis.
Keywords
Animal Experimentation
Animals
Antibodies, Fungal/*blood
Antibody Formation/*immunology
Antigens, Fungal/immunology
Dermatomycoses/blood/*immunology
Enzyme-Linked Immunosorbent Assay
Guinea Pigs
Immunity, Cellular/*immunology
Metalloproteases/analysis/*immunology
Microsporum/*enzymology/immunology
Peptide Hydrolases/metabolism
Recombinant Proteins/immunology
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 16:47
Last modification date
20/08/2019 14:30