Mycobacterium tuberculosis-specific CD8+ T cells are functionally and phenotypically different between latent infection and active disease.
Details
Serval ID
serval:BIB_7269592AD615
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Mycobacterium tuberculosis-specific CD8+ T cells are functionally and phenotypically different between latent infection and active disease.
Journal
European Journal of Immunology
ISSN
1521-4141 (Electronic)
ISSN-L
0014-2980
Publication state
Published
Issued date
2013
Volume
43
Number
6
Pages
1568-1577
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Abstract
Protective immunity to Mycobacterium tuberculosis (Mtb) remains poorly understood and the role of Mtb-specific CD8(+) T cells is controversial. Here we performed a broad phenotypic and functional characterization of Mtb-specific CD8(+) T cells in 326 subjects with latent Mtb infection (LTBI) or active TB disease (TB). Mtb-specific CD8(+) T cells were detected in most (60%) TB patients and few (15%) LTBI subjects but were of similar magnitude. Mtb-specific CD8(+) T cells in LTBI subjects were mostly T EMRA cells (CD45RA(+) CCR7(-)), coexpressing 2B4 and CD160, and in TB patients were mostly TEM cells (CD45RA(-) CCR7(-)), expressing 2B4 but lacking PD-1 and CD160. The cytokine profile was not significantly different in both groups. Furthermore, Mtb-specific CD8(+) T cells expressed low levels of perforin and granulysin but contained granzymes A and B. However, in vitro-expanded Mtb-specific CD8(+) T cells expressed perforin and granulysin. Finally, Mtb-specific CD8(+) T-cell responses were less frequently detected in extrapulmonary TB compared with pulmonary TB patients. Mtb-specific CD8(+) T-cell proliferation was also greater in patients with extrapulmonary compared with pulmonary TB. Thus, the activity of Mtb infection and clinical presentation are associated with distinct profiles of Mtb-specific CD8(+) T-cell responses. These results provide new insights in the interaction between Mtb and the host immune response.
Keywords
Acute Disease, Antigens, CD/metabolism, CD8-Positive T-Lymphocytes/immunology, Cell Proliferation, Cells, Cultured, Cytokines/metabolism, GPI-Linked Proteins/metabolism, Humans, Immunophenotyping, Latent Tuberculosis/immunology, Mycobacterium tuberculosis/immunology, Programmed Cell Death 1 Receptor/metabolism, Receptors, Immunologic/metabolism, T-Lymphocyte Subsets/immunology, Tuberculosis, Pulmonary/immunology
Pubmed
Web of science
Create date
24/06/2013 14:48
Last modification date
20/08/2019 15:30