A monoclonal antibody specific for the carboxy-terminus of angiotensin II
Details
Serval ID
serval:BIB_723C950F5818
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A monoclonal antibody specific for the carboxy-terminus of angiotensin II
Journal
Hybridoma
ISSN
0272-457X (Print)
Publication state
Published
Issued date
1984
Volume
3
Number
4
Pages
373-6
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Winter
Research Support, Non-U.S. Gov't --- Old month value: Winter
Abstract
A monoclonal antibody specific for the carboxy-terminus of angiotensin II was produced from the somatic cell fusion between SP2/0 myeloma cells and spleen cells from CAF/J mice immunized with angiotensin II selectively coupled to thyroglobulin. The subcloned line D5-7R2 was augmented in the ascites form and shown to be specific for angiotensin II (octapeptide) and shorter angiotensin peptides. This antibody, however, did not cross-react with angiotensin I (decapeptide). This selectivity will be important in clinical assays that measure angiotensin II in the presence of angiotensin I.
Keywords
Angiotensin II/*immunology
Animals
Antibodies, Monoclonal/*immunology
Antibody Specificity
Cross Reactions
Hybridomas/immunology
Immunization
Mice
Mice, Inbred Strains/immunology
Peptide Fragments/immunology
Pubmed
Web of science
Create date
05/03/2008 16:41
Last modification date
20/08/2019 14:30