Marginal role of von Willebrand factor-binding protein and coagulase in the initiation of endocarditis in rats with catheter-induced aortic vegetations.

Details

Serval ID
serval:BIB_714C89A05524
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Marginal role of von Willebrand factor-binding protein and coagulase in the initiation of endocarditis in rats with catheter-induced aortic vegetations.
Journal
Virulence
Author(s)
Mancini S., Oechslin F., Menzi C., Que Y.A., Claes J., Heying R., Veloso T.R., Vanassche T., Missiakas D., Schneewind O., Moreillon P., Entenza J.M.
ISSN
2150-5608 (Electronic)
ISSN-L
2150-5594
Publication state
Published
Issued date
2018
Peer-reviewed
Oui
Volume
9
Number
1
Pages
1615-1624
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Staphylococcus aureus is the leading cause of infective endocarditis (IE). While the role of S. aureus cell-wall associated protein clumping factor A (ClfA) in promoting IE has been already demonstrated, that of the secreted plasma-clotting factors staphylocoagulase (Coa) and von Willebrand factor-binding protein (vWbp) has not yet been elucidated. We investigated the role of Coa and vWbp in IE initiation in rats with catheter-induced aortic vegetations, using Lactococcus lactis expressing coa, vWbp, clfA or vWbp/clfA, and S. aureus Newman Δcoa, ΔvWbp, ΔclfA or Δcoa/ΔvWbp/ΔclfA mutants. vWbp-expression increased L. lactis valve infection compared to parent and coa-expressing strains (incidence: 62%, versus 0% and 13%, respectively; P < 0.01). Likewise, expression of clfA increased L. lactis infectivity (incidence: 80%), which was not further affected by co-expression of vWbp. In symmetry, deletion of the coa or vWbp genes in S. aureus did not decrease infectivity (incidence: 68 and 64%, respectively) whereas deletion of clfA did decrease valve infection (incidence: 45%; P = 0.03 versus parent), which was not further affected by the triple deletion Δcoa/ΔvWbp/ΔclfA (incidence: 36%; P > 0.05 versus ΔclfA mutant). Coa does not support the initial colonization of IE (in L. lactis) without other key virulence factors and vWbp contributes to initiation of IE (in L. lactis) but is marginal in the present of ClfA.
Keywords
Animals, Aortic Valve/microbiology, Aortic Valve/physiopathology, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Catheter-Related Infections/microbiology, Coagulase/genetics, Coagulase/metabolism, Endocarditis, Bacterial/pathology, Female, Gene Deletion, Lactococcus lactis/genetics, Lactococcus lactis/metabolism, Rats, Rats, Wistar, Staphylococcal Infections, Staphylococcus aureus/genetics, Staphylococcus aureus/pathogenicity, Virulence Factors/genetics, von Willebrand Factor/metabolism, Clumping factor A, Staphylococcus aureus, endocarditis, staphylocoagulase, von Willebrand factor-binding protein
Pubmed
Web of science
Open Access
Yes
Create date
16/10/2018 13:11
Last modification date
20/08/2019 15:29
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