Absence of ectopic epithelial inclusions in 3,904 axillary lymph nodes examined in sentinel technique.

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Serval ID
serval:BIB_6F28649497D4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Absence of ectopic epithelial inclusions in 3,904 axillary lymph nodes examined in sentinel technique.
Journal
Breast Cancer Research and Treatment
Author(s)
Iken S., Schmidt M., Braun C., Valentino A., Lehr H.A., Schaefer S.C.
ISSN
1573-7217 (Electronic)
ISSN-L
0167-6806
Publication state
Published
Issued date
12/2011
Peer-reviewed
Oui
Volume
-
Number
-
Pages
-
Language
english
Abstract
Intraoperative examination of sentinel axillary lymph nodes can be done by imprint cytology, frozen section, or, most recently, by PCR-based amplification of a cytokeratin signal. Using this technique, benign epithelial inclusions, representing mammary tissue displaced along the milk line, will likely generate a positive PCR signal and lead to a false-positive diagnosis of metastatic disease. To better appreciate the incidence of ectopic epithelial inclusions in axillary lymph nodes, we have performed an autopsy study, examining on 100 μm step sections 3,904 lymph nodes obtained from 160 axillary dissections in 80 patients. The median number of lymph nodes per axilla was 23 (15, 6, and 1 in levels 1, 2, and 3, respectively). A total of 30,450 hematoxylin-eosin stained slides were examined, as well as 8,825 slides immunostained with pan-cytokeratin antibodies. Despite this meticulous work-up, not a single epithelial inclusion was found in this study, suggesting that the incidence of such inclusions is much lower than the assumed 5% reported in the literature.
Pubmed
Web of science
Open Access
Yes
Create date
27/12/2011 10:04
Last modification date
01/10/2019 7:18
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