Survival-promoting activity of inhibitors of cyclin-dependent kinases on primary neurons correlates with inhibition of c-Jun kinase-1.

Details

Serval ID
serval:BIB_6E07FE428F4C
Type
Article: article from journal or magazin.
Collection
Publications
Title
Survival-promoting activity of inhibitors of cyclin-dependent kinases on primary neurons correlates with inhibition of c-Jun kinase-1.
Journal
Neurobiology of Disease
Author(s)
Markus M.A., Kahle P.J., Winkler A., Horstmann S., Anneser J.M., Borasio G.D.
ISSN
0969-9961 (Print)
ISSN-L
0969-9961
Publication state
Published
Issued date
1997
Volume
4
Number
2
Pages
122-133
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Abstract
Cyclin-dependent kinases and mitogen-activated protein kinases have been implicated in the regulation of cellular survival and apoptosis. We tested the effect of two mitogen-activated/cyclin-dependent kinase inhibitors, olomoucine and butyrolactone I, on the in vitro survival of chick embryonic neurons. Sensory, sympathetic, and ciliary neurons, when prepared at their respective time point of programmed cell death, could be rescued from apoptosis by both inhibitors in a dose-dependent fashion. In contrast, dividing sympathetic precursors underwent apoptosis when treated with olomoucine, but not butyrolactone I, at the same range of concentration. With similar potency, olomoucine and butyrolactone I inhibited immunocomplex c-Jun kinase activity. Both substances inhibited neurite outgrowth in a dose-dependent manner; developmentally younger neurons were more sensitive to this effect than older ones. These results suggest that certain mitogen-activated/cyclin-dependent kinases associated with cell division in neuronal precursors (i) may become essential components of the apoptotic machinery by the time neurons reach their phase of naturally occurring cell death and (ii) may be necessary for neurite outgrowth during development.
Keywords
Animals, Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors, Cell Survival/physiology, Chick Embryo, Cyclin-Dependent Kinases/antagonists & inhibitors, JNK Mitogen-Activated Protein Kinases, Mitogen-Activated Protein Kinases, Neurons/drug effects
Pubmed
Create date
13/01/2014 16:53
Last modification date
20/08/2019 14:27
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