Transient DNA Occupancy of the SMC Interarm Space in Prokaryotic Condensin.

Details

Serval ID
serval:BIB_6B7EB2A633CA
Type
Article: article from journal or magazin.
Collection
Publications
Title
Transient DNA Occupancy of the SMC Interarm Space in Prokaryotic Condensin.
Journal
Molecular cell
Author(s)
Vazquez Nunez R., Ruiz Avila L.B., Gruber S.
ISSN
1097-4164 (Electronic)
ISSN-L
1097-2765
Publication state
Published
Issued date
25/07/2019
Peer-reviewed
Oui
Volume
75
Number
2
Pages
209-223.e6
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Multi-subunit SMC ATPases control chromosome superstructure and DNA topology, presumably by DNA translocation and loop extrusion. Chromosomal DNA is entrapped within the tripartite SMCkleisin ring. Juxtaposed SMC heads ("J heads") or engaged SMC heads ("E heads") split the SMCkleisin ring into "S" and "K" sub-compartments. Here, we map a DNA-binding interface to the S compartment of E heads SmcScpAB and show that head-DNA association is essential for efficient DNA translocation and for traversing highly transcribed genes in Bacillus subtilis. We demonstrate that in J heads, SmcScpAB chromosomal DNA resides in the K compartment but is absent from the S compartment. Our results imply that the DNA occupancy of the S compartment changes during the ATP hydrolysis cycle. We propose that DNA translocation involves DNA entry into and exit out of the S compartment, possibly by DNA transfer between compartments and DNA segment capture.
Keywords
Adenosine Triphosphatases/genetics, Adenosine Triphosphate/genetics, Bacillus subtilis/genetics, Bacterial Proteins/chemistry, Bacterial Proteins/genetics, Cell Cycle Proteins/chemistry, Cell Cycle Proteins/genetics, Chromosomes, Bacterial/genetics, DNA/chemistry, DNA/genetics, DNA-Binding Proteins/chemistry, DNA-Binding Proteins/genetics, Hydrolysis, Multiprotein Complexes/genetics, Nucleic Acid Conformation, Prokaryotic Cells/chemistry, DNA loop extrusion, MukB, Rad50, SMC, Smc5, SmcScpAB, chromosome condensation, cohesin, condensin, kleisin
Pubmed
Web of science
Create date
17/06/2019 22:44
Last modification date
30/01/2020 7:20
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