LACK-specific CD4(+) T cells that induce gamma interferon production in patients with localized cutaneous leishmaniasis during an early stage of infection.
Details
Serval ID
serval:BIB_6A2A8086D015
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
LACK-specific CD4(+) T cells that induce gamma interferon production in patients with localized cutaneous leishmaniasis during an early stage of infection.
Journal
Infection and Immunity
ISSN
0019-9567 (Print)
ISSN-L
0019-9567
Publication state
Published
Issued date
2002
Volume
70
Number
6
Pages
3122-3129
Language
english
Abstract
The profile of cytokines induced by soluble leishmania antigen (SLA) and the Leishmania homologue of the mammalian receptor for activated C kinase (LACK), a candidate vaccine against leishmaniasis, and the cellular source of the cytokines produced in response to these antigens were analyzed in patients infected with Leishmania guyanensis. Gamma interferon (IFN-gamma) and interleukin-10 (IL-10) were produced in response to LACK. Although LACK-specific CD4(+) cells producing IFN-gamma were isolated only during the early phase of infection (less than 30 days following the onset of infection), cells producing IL-10 in response to LACK were detected in all patients. CD4(+) T cells producing IFN-gamma and IL-13 were produced in response to SLA in all patients. SLA- and LACK-specific T cells are effector memory cells, as they are CD45RA(-) CCR7(-) CD4(+) T cells. CD4(+) T cells producing IFN-gamma are CD62L(-), and CD4(+) T cells producing IL-10 are CD62L(+), indicating that these cells have different tissue-homing capacities. These findings show that SLA and LACK induce both type 1 (IFN-gamma) and type 2 (IL-10 or IL-13) cell responses.
Keywords
Amino Acid Sequence, Animals, Antigens, CD45, Antigens, Protozoan/immunology, Biological Markers, CD4-Positive T-Lymphocytes/immunology, Cells, Cultured, Humans, Interferon-gamma/biosynthesis, Interleukin-10/secretion, Interleukin-13/biosynthesis, L-Selectin, Leishmania guyanensis/immunology, Leishmaniasis, Mucocutaneous/blood, Leishmaniasis, Mucocutaneous/immunology, Leukocytes, Mononuclear/cytology, Leukocytes, Mononuclear/immunology, Molecular Sequence Data, Peptides/immunology, Protein Tyrosine Phosphatase, Non-Receptor Type 1, Protozoan Proteins/immunology, Receptors, CCR7, Receptors, Chemokine, Time Factors
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 12:06
Last modification date
20/08/2019 15:24