Separation of blood microsamples by exploiting sedimentation at the microscale.

Details

Ressource 1Download: pmid30237536.pdf (2263.55 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_6A1C20674E95
Type
Article: article from journal or magazin.
Collection
Publications
Title
Separation of blood microsamples by exploiting sedimentation at the microscale.
Journal
Scientific reports
Author(s)
Forchelet D., Béguin S., Sajic T., Bararpour N., Pataky Z., Frias M., Grabherr S., Augsburger M., Liu Y., Charnley M., Déglon J., Aebersold R., Thomas A., Renaud P.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
20/09/2018
Peer-reviewed
Oui
Volume
8
Number
1
Pages
14101
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Microsample analysis is highly beneficial in blood-based testing where cutting-edge bioanalytical technologies enable the analysis of volumes down to a few tens of microliters. Despite the availability of analytical methods, the difficulty in obtaining high-quality and standardized microsamples at the point of collection remains a major limitation of the process. Here, we detail and model a blood separation principle which exploits discrete viscosity differences caused by blood particle sedimentation in a laminar flow. Based on this phenomenon, we developed a portable capillary-driven microfluidic device that separates blood microsamples collected from finger-pricks and delivers 2 µL of metered serum for bench-top analysis. Flow cytometric analysis demonstrated the high purity of generated microsamples. Proteomic and metabolomic analyses of the microsamples of 283 proteins and 1351 metabolite features was consistent with samples generated via a conventional centrifugation method. These results were confirmed by a clinical study scrutinising 8 blood markers in obese patients.
Pubmed
Web of science
Open Access
Yes
Create date
24/09/2018 8:24
Last modification date
20/08/2019 14:24
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