Experimental and computational approaches to quantitative proteomics: status quo and outlook.
Details
Serval ID
serval:BIB_699FD94D1B7A
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Experimental and computational approaches to quantitative proteomics: status quo and outlook.
Journal
Journal of Proteomics
ISSN
1874-3919 (Print)
Publication state
Published
Issued date
2008
Volume
71
Number
1
Pages
19-33
Language
english
Notes
Publication types: Journal Article ; Review
Publication Status: ppublish
Publication Status: ppublish
Abstract
Proteomics has come a long way from the initial qualitative analysis of proteins present in a given sample at a given time ("cataloguing") to large-scale characterization of proteomes, their interactions and dynamic behavior. Originally enabled by breakthroughs in protein separation and visualization (by two-dimensional gels) and protein identification (by mass spectrometry), the discipline now encompasses a large body of protein and peptide separation, labeling, detection and sequencing tools supported by computational data processing. The decisive mass spectrometric developments and most recent instrumentation news are briefly mentioned accompanied by a short review of gel and chromatographic techniques for protein/peptide separation, depletion and enrichment. Special emphasis is placed on quantification techniques: gel-based, and label-free techniques are briefly discussed whereas stable-isotope coding and internal peptide standards are extensively reviewed. Another special chapter is dedicated to software and computing tools for proteomic data processing and validation. A short assessment of the status quo and recommendations for future developments round up this journey through quantitative proteomics.
Keywords
Computational Biology/methods, Computational Biology/trends, Humans, Proteins/isolation & purification, Proteomics/instrumentation, Proteomics/methods
Pubmed
Web of science
Create date
29/01/2009 23:13
Last modification date
20/08/2019 15:24