Stereospecific recognition of pyochelin and enantio-pyochelin by the PchR proteins in fluorescent pseudomonads.

Details

Serval ID
serval:BIB_63BA28F2E25F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Stereospecific recognition of pyochelin and enantio-pyochelin by the PchR proteins in fluorescent pseudomonads.
Journal
Microbiology
Author(s)
Youard Z.A., Reimmann C.
ISSN
1465-2080[electronic], 1350-0872[linking]
Publication state
Published
Issued date
2010
Peer-reviewed
Oui
Volume
156
Number
6
Pages
1772-1782
Language
english
Abstract
The siderophore pyochelin of Pseudomonas aeruginosa promotes growth under iron limitation and induces the expression of its biosynthesis genes via the transcriptional AraC/XylS-type regulator PchR. Pseudomonas fluorescens strain CHA0 makes the optical antipode of pyochelin termed enantio-pyochelin, which also promotes growth and induces the expression of its biosynthesis genes when iron is scarce. Growth promotion and signalling by pyochelin and enantio-pyochelin are highly stereospecific and are known to involve the pyochelin and enantio-pyochelin outer-membrane receptors FptA and FetA, respectively. Here we show that stereospecificity in signalling is also based on the stereospecificity of the homologous PchR proteins of P. aeruginosa and P. fluorescens towards their respective siderophore effectors. We found that PchR functioned in the heterologous species only if supplied with its native ligand and that the FptA and FetA receptors enhanced the efficiency of signalling. By constructing and expressing hybrid and truncated PchR regulators we showed that the weakly conserved N-terminal domain of PchR is responsible for siderophore specificity. Thus, both uptake and transcriptional regulation confer stereospecificity to pyochelin and enantio-pyochelin biosynthesis.
Keywords
Amino Acid Sequence, Bacterial Proteins/chemistry, Bacterial Proteins/metabolism, DNA-Binding Proteins/chemistry, DNA-Binding Proteins/metabolism, Iron/metabolism, Ligands, Molecular Sequence Data, Phenols/chemistry, Pseudomonas aeruginosa/genetics, Pseudomonas aeruginosa/metabolism, Pseudomonas fluorescens/metabolism, Siderophores/metabolism, Thiazoles/chemistry, Transcription Factors/chemistry, Transcription Factors/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
11/01/2011 14:18
Last modification date
20/08/2019 14:20
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