Sensory neuron lineage mapping and manipulation in the Drosophila olfactory system.

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Serval ID
serval:BIB_616D5C56C195
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Sensory neuron lineage mapping and manipulation in the Drosophila olfactory system.
Journal
Nature Communications
Author(s)
Chai P.C., Cruchet S., Wigger L., Benton R.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
07/02/2019
Peer-reviewed
Oui
Volume
10
Number
1
Pages
643
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Nervous systems exhibit myriad cell types, but understanding how this diversity arises is hampered by the difficulty to visualize and genetically-probe specific lineages, especially at early developmental stages prior to expression of unique molecular markers. Here, we use a genetic immortalization method to analyze the development of sensory neuron lineages in the Drosophila olfactory system, from their origin to terminal differentiation. We apply this approach to define a fate map of nearly all olfactory lineages and refine the model of temporal patterns of lineage divisions. Taking advantage of a selective marker for the lineage that gives rise to Or67d pheromone-sensing neurons and a genome-wide transcription factor RNAi screen, we identify the spatial and temporal requirements for Pointed, an ETS family member, in this developmental pathway. Transcriptomic analysis of wild-type and Pointed-depleted olfactory tissue reveals a universal requirement for this factor as a switch-like determinant of fates in these sensory lineages.
Keywords
Animals, Drosophila, Drosophila Proteins/metabolism, Olfactory Bulb/cytology, Olfactory Bulb/metabolism, Sensory Receptor Cells/cytology, Sensory Receptor Cells/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
01/05/2018 16:57
Last modification date
26/11/2019 7:19
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