CRISPR-based gene expression control for synthetic gene circuits

Details

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State: Public
Version: author
License: CC BY 4.0
Serval ID
serval:BIB_5D99E1081188
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
CRISPR-based gene expression control for synthetic gene circuits
Journal
Biochemical Society Transactions
Author(s)
Santos-Moreno Javier, Schaerli Yolanda
ISSN
0300-5127
1470-8752
ISSN-L
0300-5127
Publication state
Published
Issued date
30/10/2020
Peer-reviewed
Oui
Volume
48
Number
5
Pages
1979-93
Language
english
Notes
Publication types: Journal Article
Publication Status: aheadofprint
Abstract
Synthetic gene circuits allow us to govern cell behavior in a programmable manner, which is central to almost any application aiming to harness engineered living cells for user-defined tasks. Transcription factors (TFs) constitute the 'classic' tool for synthetic circuit construction but some of their inherent constraints, such as insufficient modularity, orthogonality and programmability, limit progress in such forward-engineering endeavors. Here we review how CRISPR (clustered regularly interspaced short palindromic repeats) technology offers new and powerful possibilities for synthetic circuit design. CRISPR systems offer superior characteristics over TFs in many aspects relevant to a modular, predictable and standardized circuit design. Thus, the choice of CRISPR technology as a framework for synthetic circuit design constitutes a valid alternative to complement or replace TFs in synthetic circuits and promises the realization of more ambitious designs.
Keywords
Biochemistry
Pubmed
Web of science
Open Access
Yes
Create date
26/09/2020 14:48
Last modification date
18/11/2020 6:24
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