A repeated GGA motif is critical for the activity and stability of the riboregulator RsmY of Pseudomonas fluorescens.

Details

Serval ID
serval:BIB_5D5BFDF7E684
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A repeated GGA motif is critical for the activity and stability of the riboregulator RsmY of Pseudomonas fluorescens.
Journal
Journal of Biological Chemistry
Author(s)
Valverde C., Lindell M., Wagner E.G., Haas D.
ISSN
0021-9258[print], 0021-9258[linking]
Publication state
Published
Issued date
2004
Volume
279
Number
24
Pages
25066-25074
Language
english
Abstract
The riboregulator RsmY of Pseudomonas fluorescens strain CHA0 is an example of small regulatory RNAs belonging to the global Rsm/Csr regulatory systems controlling diverse cellular processes such as glycogen accumulation, motility, or formation of extracellular products in various bacteria. By binding multiple molecules of the small regulatory protein RsmA, RsmY relieves the negative effect of RsmA on the translation of several target genes involved in the biocontrol properties of strain CHA0. RsmY and functionally related riboregulators have repeated GGA motifs predicted to be exposed in single-stranded regions, notably in the loops of hairpins. The secondary structure of RsmY was corroborated by in vivo cleavage with lead acetate. RsmY mutants lacking three or five (out of six) of the GGA motifs showed reduced ability to derepress the expression of target genes in vivo and failed to bind the RsmA protein efficiently in vitro. The absence of GGA motifs in RsmY mutants resulted in reduced abundance of these transcripts and in a shorter half-life (< or = 6 min as compared with 27 min for wild type RsmY). These results suggest that both the interaction of RsmY with RsmA and the stability of RsmY strongly depend on the GGA repeats and that the ability of RsmY to interact with small regulatory proteins such as RsmA may protect this RNA from degradation.
Keywords
Bacterial Proteins/chemistry, Base Sequence, Escherichia coli/genetics, Gene Expression Regulation, Bacterial, Molecular Sequence Data, Mutation, Nucleic Acid Conformation, Pseudomonas fluorescens/genetics, Pseudomonas fluorescens/metabolism, RNA, Bacterial/chemistry, RNA, Bacterial/physiology, RNA-Binding Proteins/chemistry, Repressor Proteins/chemistry, Trinucleotide Repeats
Pubmed
Web of science
Create date
25/01/2008 17:01
Last modification date
20/08/2019 14:15
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